Author/Authors :
Yazdi، Hamid Reza نويسنده MD, Assistant Professor, Department of Orthopedic Surgery,
Firoozgar Hospital, Iran University of Medical Science, Tehran, IR
Iran , , Jamei Moayedi، Reza نويسنده MD, Resident of Orthopedics, Department of Orthopedic
Surgery, Shafa Orthopedic Hospital, Iran University of Medical
Science, Tehran, IR Iran , , Shokrgozar، Mohammad Ali نويسنده , , Dehghan، Mohammad Mehdi نويسنده , , Mokhtari، Tahmineh نويسنده Department of Anatomy, Tehran University of Medical Sciences, Tehran, IR Iran ,
Abstract :
Background: Antibiotics are the most common additives used in irrigation solutions for open fractures. None-the less, there have been few studies exploring the effects of antibiotics on articular cartilage. The purpose of this study was to evaluate the delayed effect of intra-articular injection of cefazolin, gentamicin and vancomycin on articular cartilage.
Objectives: We proposed they may have potential toxicity on articular cartilage and this effect may be manifested as a delayed phenomenon due to changes in matrix and cell viability.
Materials and Methods: A sample of 20 rabbits was chosen and assigned into 4 groups (each containing 5 animals). A course of antibiotics including gentamicin, vancomycin and cefazolin was injected in both knees of test rabbits. Normal saline was injected to controls. After 8 weeks, articular cartilage of distal femurs was harvested and analyzed using confocal microscopy and Real time PCR.
Results: According to confocal microscopy results, gentamicin and vancomycin had significant decrease in live chondrocyte cell number; however, cefazolin had no effect.
Conclusions: Quantitative analysis showed no significant difference between antibiotics and control group. We found that cefazolin can be safely used for intra-articular injections, but vancomycin and gentamicin should be used cautiously.
