Author/Authors :
Kim، HS نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Kim, HS , Lee، H نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Lee, H , Kim، HS نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Kim, HS , Won، Sulmui نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Won, Sulmui , Lee، EK نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Lee, EK , Bang، Kyongwon نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Bang, Kyongwon , Chun، YH نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Chun, YH , Yoon، Jongseo نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Yoon, Jongseo , Kim، HH نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Kim, HH , Kim، Jin Tack نويسنده Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Kim, Jin Tack
Abstract :
Respiratory virus infection is a major cause of asthma exacerbation. However, the underlying mechanisms of this exacerbation are unknown. Therefore, to determine the mechanisms, we examined the effect of influenza infection in a murine model of asthma.Mice were divided into four groups: the phosphate-buffered saline (PBS), house dust mite (HDM), influenza, and HDM/influenza groups. The influenza group and the HDM/influenza group were infected with influenza A virus. We measured airway resistance (Penh value), examined the lung tissue for pathology, and analyzed the cells and cytokines in bronchoalveolar lavage fluid (BALF) by ELISA.At 50 mg/mL methacholine, the HDM/influenza group showed a significantly higher Penh value than the PBS, HDM, and influenza groups. The number of neutrophils in BALF was higher in the HDM/influenza group than in the HDM group. A significantly greater number of lymphocytes and macrophages were detected in the HDM/influenza group than in the HDM group. IFN-γ and IL-1β levels were higher in the HDM/influenza group than in the HDM group. IL-5 levels did not vary between the HDM and HDM/influenza groups, IL-10 was significantly lower in the HDM/influenza than in the HDM group. Chemokine (C-X-C motif) ligand 1 (CXCL1) and regulated upon activation, normal T cell expressed and secreted (RANTES) were higher in the HDM/influenza group than in the HDM group.In a murine model of asthma, influenza-induced airway inflammation appeared to becaused by simultaneous activation of neutrophilic and eosinophilic inflammation.
