Author/Authors :
Fatemi، Iman نويسنده Department of Pharmacology, School of Medicine and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran Fatemi, Iman , Shamsizadeh، Ali نويسنده , , Roohbakhsh، Ali نويسنده Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran , , Ayoobi، Fatemeh نويسنده Geriatic Care Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Ayoobi, Fatemeh , Sanati، Mohammad Hossein نويسنده , , Motevalian، Manijeh نويسنده Department of Pharmacology, School of Medicine and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran Motevalian, Manijeh
Abstract :
Orexin A and B are hypothalamic peptides with a wide variety of effects such as anti-inflammation and neuroprotection. Impaired function of orexin system has been reported in some neurodegenerative diseases like Parkinson, Huntington and Alzheimer. In this study, the mRNA expression levels of some hypothalamic peptides were investigated in C57BL/6 female mice with experimental autoimmune encephalomyelitis (EAE). Animals were randomly divided into two control and EAE groups. EAE was induced by administration of myelin oligodendrocyte glycoprotein (MOG) with complete Ferund’s adjuvant and pertussis toxin. Twenty-first days following immunization, mice were decapitated to remove the brains. Then, the expression profiles of prepro-orexin, orexin 1 receptors (OX1R) and orexin 2 receptors (OX2R) in hypothalamic region were assessed using real-time PCR method. In this study, we found a considerable increase in the mRNA expression of OX1R and OX2R following EAE induction in C57BL/6 mice. Elevation levels of OX1R and OX2R following EAE induction suggest that alteration in orexinergic system may involve in pathogenesis of multiple sclerosis.
