Author/Authors :
سخاوتي، م.ه. نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Sekhavati, M.H. , طهمورث?پور، م. نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Tahmoorespur, M. , عباسي-دلوئي، ط. نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Abbassi-Daloii, T. , يوسفي، س. نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Yousefi, S. , خبيري، ع.ا. نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Khabiri, A.A. , اکبري، ر. نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Akbari, R. , شکاري، ع نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Shekari, E.
Abstract :
There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrated for construction of two-promoter vector to express two genes equally. To construct two-promoter vector, two pairs of mega-primer containing enzymatic restriction sites, T7 promoter, Lac operator and ribosome binding site (RBS) sequences were used in SOE-PCR. The constructed vector can be used in order to co-expression of other genes properly in a variety of bacterial expression hosts.
