Author/Authors :
Moghadam,، R . Golijani نويسنده National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, Iran , , Samani، M Yazdanpanah نويسنده National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, Iran , , Zamani، M R نويسنده National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, Iran , , Motallebi، M نويسنده National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, Iran ,
Abstract :
An endochitinase gene (chit36) was isolated from the biocontrol fungus Trichoderma atroviride. Chit36 was
overexpressed under the CaMV35S constitutive promoter in canola. Transformation of cotyledonary petioles was
achieved via Agrobacterium tumefaciens. The insertion of transgene was verified by PCR and DNA dot blotting.
RT-PCR analysis indicated that the transgenic canola was able to express chit36 gene. Also, we used a transgenic
over-expression approach in order to investigate antifungal activity of expressed chit36 on Sclerotinia
sclerotiorum. Antifungal activity was detected in crude protein extracts from transgenic canola using radial
diffusion assay. Also, lesion sizes of transgenic canola by S. sclerotiorum were significantly retarded compared to
non transgenic canola plant using detached leaf assay.
