Author/Authors :
Assadollahi، Vahideh نويسنده Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, IR Iran , , Gholami، Mohammadreza نويسنده , , Zendedel، Abolfazl نويسنده Department of Internist, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR Iran , , Afsartala، Zohreh نويسنده Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, IR Iran , , Jahanmardi، Ferdoos نويسنده Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR Iran ,
Abstract :
Background:
Genes are involved in the control of stem cell self-renewal as a new class of molecular markers of cancer.
Objectives:
In this study, the expression of Oct4, Nanog and Sox2 in cell lines MIA Paca-2, PA-TU-8902 and AsPC-1 and pancreatic cancer tissue were examined.
Materials and Methods:
In this experimental study, cell lines, MIA Paca-2, PA-TU-8902 and AsPC-1, were cultured in DMEM (Dulbecco’s Modified Eagles Medium) and RPMI-1640 (Roswell Park Memorial Institute) containing FBS 10% (fetal bovine serum) in a 37°C incubator containing Co2 5% and humidity 90%. Samples of tumor and non-cancer pancreatic tumor were purchased Iran tumor bank. Extraction of RNA and synthesis of cDNA was performed. Expression levels of Oct4, Nanog and Sox2 were determined using Real-time PCR. The protein expression levels of target genes in the cell lines were studied by flow cytometry and immunocytochemistry.
Results:
The expression rate of Oct4, Nanog and Sox2 is more in the cancer cell lines than those in the control (normal tissue) samples. The protein expression levels of target genes in the cell lines were confirmed by flow cytometry and immunocytochemistry.
Conclusions:
The genes are involved in stem cell self-renewal as a new class of molecular markers of cancer that detected in the pancreatic cell lines. Maybe, these genes play important role in the uncontrolled proliferation of cancer cells.
