Author/Authors :
Kachuei ، R نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Scie nces, Tehran, Iran , , Yadegari، MH نويسنده Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran , , Safaie ، N نويسنده Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran , , Ghiasian ، A نويسنده Medical Parasitology and Mycology De partment, School of Medicine, Hamadan University of Medical Sciences and Health Services, Hamadan, Iran , , Noorbakhsh ، F نويسنده Department of Biology, School of Basic Science , Islamic Azad University - Pishva Varamin Branch, Tehran, Iran , , Piranfar ، V نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Scie nces, Tehran, Iran , , Rezaie ، S نويسنده Department of Medical Parasitology and My cology, Faculty of Public Health, Tehran University of Medial Sciences, Iran ,
Abstract :
Background and Purpose:
The
Fusarium
species are among the most important fungi in the medical, veterinary and
a
gricultural fields.
Materials and Methods
:
In the present study, 172 strains of these fungi have been analyzed. The high molecular
weight DNAs were extracted from 23 reference strains as well as from 149 isolated
Fusarium
species. Using
the
designed nucle
otide primers from
rDNA of
Fusarium
species
,
PCR analysis w
as
performed for the amplification of
ITS
regions
.
Afterwards, the location of the effective endonuclease enzymes has been evaluated within approximately 930
bp of
rDNA
sequence.
Results
:
Through
the selected enzymes including;
Hha
I,
Msp
I,
Taq
I and
Faq
I, the mentioned
Fusarium
species have
been divided into 33 groups
.
The first three enzymes were able to classify
Fusarium
species into 23 groups of which
19
groups included one member, one group incl
uded two members and three groups included three members of the
Fusarium
species
. This study also revealed the possibility in
the
identification of
F. semitectum
,
F. solani
complex
,
F.
pseudograminearum
,
F. nisikadoi
,
F. coeruleum
and
F. acuminatum
species
by one unique enzyme
. In addition, our
study indicated the ability of the differentiation of
F. Compactum
from
F. equiseti
.
Conclusion:
As
Compared to previous studies
with more
endonuclease enzymes and
with limited in identifications, the
ITS
-
RFLP patter
ns reported here
an
attempted to evaluate most of
the
Fusarium
species successfully
