Author/Authors :
Nazemi ، L نويسنده Division of Molecular Biology, Department of Medical Mycology & Parasitol ogy, School of Public Health, Tehran University of Medical Sciences, Tehran , Iran , , Kordbacheh ، P نويسنده Division of Molecular Biology, Department of Medical Mycology & Parasitol ogy, School of Public Health, Tehran University of Medical Sciences, Tehran , Iran , , Daei Ghazvini ، R نويسنده Division of Molecular Biology, Department of Medical Mycology & Parasitol ogy, School of Public Health, Tehran University of Medical Sciences, Tehran , Iran , , Moazeni ، M نويسنده Invasive Fungi Research Centre/ Department of Medical Mycology & Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , , Akbari Dana، M نويسنده Division of Molecular Biology, Department of Medical Mycology & Parasitol ogy, School of Public Health, Tehran University of Medical Sciences, Tehran , Iran , , Rezaie ، S نويسنده Division of Molecular Biology, Department of Medical Mycology & Parasitol ogy, School of Public Health, Tehran University of Medical Sciences, Tehran , Iran ,
Abstract :
Background and Purpose:
Mycotoxins are secondary fungal metabolites with a
very high diversity that are
produced by some species of
Aspergillus
which frequently leads to contaminate food and agricultural products.
Recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide.
Althou
gh, the antibacterial and antifungal effects of vitamins as natural compounds have been proven, the
mechanism of vitamins effect on
Aspergillus parasiticus
growth and aflatoxin production is not yet clear. In this
study, the effect of thiamine (vitamin B
1
)
was studied on
Aspergillus parasiticus
growth, aflatoxins production and
the
afIR
gene expression
.
Materials and Methods:
A standard strain of
Aspergillus parasiticus
was applied for performing antifungal
susceptibility test in different concentrations of
thiamine. Antifungal susceptibility test was performed according
to
CLSI M38
-
A2 document. The concentration of aflatoxin was determined by HPLC. Moreover, the quantitative
changes in the aflR gene expression were analyzed by
Real Time PCR method
.
Results
:
The minimum inhibitory concentration was yielded as > 500 mg/ml. However, HPLC analysis results
showed that aflatoxin production reduced in samples treated with 500 mg/ml of thiamine. In addition, the level of
afIR
gene expression was significantly reduc
ed after treating with 500 and 250 mg/ml of
vitamin B
1
.
Conclusion:
Based on the obtained results, thiamine could not inhibit the fungal growth completely. However, the
rate of
afIR
gene expression and aflatoxin production was significantly reduced after f
ungal treating with
thiamine.
Consequently, using natural compounds such as vitamins may be regarded as potential antitoxic agent in
food industry and the industries related to agriculture
