Author/Authors :
Parian ، M نويسنده Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Fata ، A نويسنده Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Najafzadeh ، MJ نويسنده Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Rezaeitalab، F نويسنده Department of Internal Medici ne, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ,
Abstract :
Background and Purpose:
Pneumocystis pneumonia, caused by
Pneumocystis jirovecii
, is a fatal disease threatening
patients with AIDS or immunosuppression. Assessment of colonization in these patients is of great significance, since it
can lead to severe pulmonary disorders. Considering the scarcity of published reports on
Pneumocystis jirovecii
isolates
from patients in Mashhad, Iran, we aimed to evaluate pneumoc
ystis colonization in individuals with different pulmonary
disorders.
Materials and Methods:
We used nested polymerase chain reaction (PCR) method to amplify mitochondrial large
subunit-ribosomal ribonucleic acid (mtLSU-rRNA) gene in
60 bronchoalveolar lavage (BAL) samples, obtained from
patients, referring to the Depa
rtment of Internal Medicine
(Pulmonary Diseases Section) at Imam Reza Hospital,
affiliated to Mashhad University of Medical Sciences, Mashhad, Iran.
Results:
DNA of
Pneumocystis jirovecii
was detected in 10 out of 60 BAL samples (16.66%) via nested PCR method.
Conclusion:
According to the present findings, the colonization rate of
Pneumocystis jirovecii
was similar to the rates
reported in other similar studies in Iran.
