Author/Authors :
Hadizadeh ، S نويسنده Department of Medical Mycology & Parasitology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran , , Forootanfar، H نويسنده Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran , , Shahidi Bonjar، G.H نويسنده Department of Plant Pathology and Biotechnology, College of Agricultural Sciences, Bahonar University of Kerman, Kerman, Iran , , Falahati Nejad ، M نويسنده Student Research Committee, Mazandaran Univ ersity of Medical Sciences, Sari, Iran , , Karamy Robati، A نويسنده Department of Medical Mycology & Parasitology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran , , Ayatollahi Mousavi ، SA نويسنده Department of Medical Mycology & Parasitology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran , , Amirporrostami ، S نويسنده Department of Medical Mycology & Parasitology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran ,
Abstract :
Background and Purpose:
Actinomycetes have been discovered as source of antifungal compounds that are currently in
clinical use. Invasive aspergillosis (IA) due to
Aspergillus fumigatus
has been identified as individual drug-resistant
Aspergillus spp.
to be an emerging pathogen opportunities a global scal
e. This paper described the antifungal activity of
one terrestrial actinomycete against th
e clinically isolated azole-resistant
A. fumigatus
.
Materials and Methods:
Soil samples were collected from various lo
cations of Kerman, Iran. Thereafter, the
actinomycetes were isolated us
ing starch-casein-nitrate-agar medium and th
e most efficient actinomycetes (capable of
inhibiting
A. fumigatus
) were screened using agar block method. In the next step, the selected actinomycete was
cultivated in starch-casein- broth medium and the inhibitory activity of the obtained culture broth was evaluated using
agar well diffusion method.
Results:
The selected actinomycete, identified as
Streptomyces rochei
strain HF391, could suppress the growth of
A.
fumigatus
isolates which was isolated from the clinical samples of patients treated with azoles. This strain showed
higher inhibition zones on agar diffusion assay which was more than 15 mm.
Conclusion:
The obtained results of the present study introduced
Streptomyces rochei
strain HF391 as terrestrial
actinomycete that can inhibit the growth of clinically isolated
A. fumigatus.
