Author/Authors :
Mahmoudi ، Mahdi نويسنده Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. , , Jamshidi، Ahmad-Reza نويسنده , , Amirzargar، Ali Akbar نويسنده , , Farhadi ، Elham نويسنده Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran. , , Nourijelyani، Keramat نويسنده , , Fallahi ، Sasan نويسنده Department of Rheumatology, Kerman University of Medical Sciences, Kerman, Iran. , , Oraei، Mona نويسنده Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Oraei, Mona , Noori، Sahar نويسنده Aerospace Research Institute, Tehran Noori, Sahar , Nicknam ، Mohammad Hossein نويسنده Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran ,
Abstract :
Ankylosing Spondylitis (AS) is an inflammatory arthritis, which affects mainly spine and sacroiliac joints. According to recent studies, ERAP1 is the second most common candidate gene for AS susceptibility after HLA-B27. The aim of this study was to determine the association of ERAP1 gene polymorphisms with AS in Iranian population.
The study group comprised 387 Iranian AS patients and 316 healthy controls from Iran.
Using Real Time PCR allelic discrimination method, we genotyped four SNPs (rs30187, rs469876, rs13167972 and rs27434) of ERAP1.
We found that rs30187 and rs27434 were significantly associated with AS in Iranian population (P=6×10-5, P=7×10-3, respectively). The rs30187 T/T genotype was associated with AS compared with C/C genotype (P=1.5×10-5). The rs27434 G/G genotype was inversely associated with AS (P=5×10-3). Two specific haplotypes including: rs30187/ rs469876/ rs13167972/ rs27434 TAAA and CAGG were associated with increased and decreased risk of AS in Iranian population, respectively.
These results indicated that ERAP1 SNPs and haplotypes were associated with AS in Iranian population.
