Author/Authors :
Gholami، Mohammadreza نويسنده , , Saki، Ghasem نويسنده Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , , Hemadi، Masoud نويسنده Associate Professor, Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , , Khodadadi، Abbas ali نويسنده , , Mohammadiasl، Javad نويسنده Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ,
Abstract :
Background: Freezing of the immature testis tissue and then its transplantation can be considered a major step in fertility preservation for young boys with cancer.
Objectives: The objective of this study was to investigate the antioxidant effects of melatonin on histological structure of seminiferous tubules that were vitrified-thawed with different cryoprotectant media.
Materials and Methods: Male mice were randomly assigned to one of 10 experimental groups. Testes of 6-day-old BALB/c male mice pups were extracted and vitrified-thawed with different vitrification media with melatonin and without melatonin. Then, their testes were stained with haematoxylin-eosin.
Results: The result showed that supplementation of glycerol based media with melatonin has reduced injury to histological structure of seminiferous tubules in the 6-day-old male BALB/c mice pups testes (P = 0.004).
Conclusions: It seem that melatonin due to its antioxidant properties, if added to glycerol based media, reduces the harmful effects of freezing-thawing process, and protects seminiferous tubules histological structure from oxidative injury.
