Author/Authors :
LI، Jianguo نويسنده , , Zhuan-sun، Yong-xun نويسنده Department of Respirology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Respiratory Disease Research Institute of Sun Yat-Sen University, Guangzhou, Guangdong province, People’s Republic of China Zhuan-sun, Yong-xun , Wen، Shaw-Bing نويسنده , , Wu، Guang-Hao نويسنده , , Huang، Feng-ting نويسنده Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Respiratory Disease Research Institute of Sun Yat-Sen University, Guangzhou, Guangdong province, People’s Republic of China Huang, Feng-ting , Ghimire، Hridaya bibhu نويسنده Department of Respirology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Respiratory Disease Research Institute of Sun Yat-Sen University, Guangzhou, Guangdong province, People’s Republic of China Ghimire, Hridaya bibhu , Ran، Pi-xin نويسنده Department of Respirology, The First Affiliated Hospital of Guang Zhou Medical College, Guangzhou, Guangdong province, People’s Republic of China Ran, Pi-xin
Abstract :
Up-regulation of CD4+CD25+CD127low/- regulatory T cells (Tregs) is a new target in the treatment of asthma. Human bone marrow mesenchymal stem cells can up-regulate CD4+CD25+CD127low/- regulatory T cells in vitro, meanwhile, heme oxygenase-1 (HO-1) plays an important role in the development and maintenance of CD4+CD25+ regulatory T cells. However the mechanism has not yet been adequately understood. Hence, we wondered what effect of Heme Oxygenase-1 made on regulation of CD4+CD25+CD127low/- regulatory T cells mediated by mesenchymal stem cells.
Peripheral blood mononuclear cells isolated from asthmatic patients and healthy controls were co-cultured with human bone marrow mesenchymal stem cells which were pretreated with Hemin (the revulsive of Heme Oxygenase-1), Protoporphyrin Ⅸ zinc (the inhibitor of Heme Oxygenase-1) and saline.
The expression of Heme Oxygenase-1 in MSCs was enhanced by Hemin and inhibited by Protoporphyrin zinc in vitro. Overexpression of Heme Oxygenase-1 elevated the proportion of CD4+CD25+CD127low/- regulatory T cells in CD4+ T cells, meanwhile, inhibition of Heme Oxygenase-1 decreased the proportion of CD4+CD25+CD127low/- regulatory T cells in CD4+ T cells as compared with mesenchymal stem cells alone.
Taken together, these data demonstrated that Heme Oxygenase-1 contributed to the up-regulation of CD4+CD25+CD127low/- regulatory T cells mediated by mesenchymal stem cells in asthma.
