Histological assessment of pulpal responses to resin modifi ed glass ionomer cements in human teeth

Background: The biocompatibility of resin-modifi ed glass ionomers (RMGIs) as a lining material is still under question. The present study evaluated the response of the pulp-dentin complex following application of resin-modifi ed glass-ionomer cement, calcium hydroxide and conventional glass-ionomer in deep cavities prepared in human teeth. Materials and Methods: In this controlled clinical trial, 30 deep class V buccal cavities (3 mm × 2 mm × 2 mm) were prepared in human premolars treatment planned to be extracted for orthodontic reasons and divided into 3 groups. Groups were lined by a RMGI (Vivaglass), conventional glass Ionomer (Ionocid) and calcium hydroxide respectively. The cavities were subsequently fi lled with amalgam. Each group was then divided into two sub-groups according to time intervals 5 and 30 days. The patients were referred to Kerman Dental School and in accordance with orthodontic treatment plan; premolars were extracted and then prepared for histological assessment. The sections were stained with hematoxylin and eosin and periodic acid Schiff techniques. All of the samples were examined using a number of criteria including odontoblastic changes, infl ammatory cells response, reactionary dentin formation and presence of microorganisms. The data were analyzed by Kruskal-Wallis and Mann-Whitney tests. P < 0.05 was considered as signifi cant. Results: There was no signifi cant difference among odontoblastic changes, reactionary dentin, presence of bacteria and infl ammatory cells response of the groups (P > 0.05). Conclusion: Ionocid and Vivaglass resin-modifi ed glass ionomers can be used as lining materials in human teeth.