Author/Authors :
Haji Abbas Tabrizi، Fatemeh نويسنده 1Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, I.R. Iran. , , Irian، Saeed نويسنده Department of Biology, Faculty of Science , , Amanzadeh، Amir نويسنده National Cell Bank of Iran, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran , , Heidarnejad، Fatemeh نويسنده Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran , , Gudarzi، Hoda نويسنده 3Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, I.R. Iran. , , Salimi، Mona نويسنده ,
Abstract :
Hoda GudarziPlant-derived natural products are known to have cancer chemo-preventive and chemo-therapeutic properties. Plant extracts or their active constituents are used as folk medicine in traditional therapies by 80% of the world population. The aim of the present study was to determine the anti-proliferative potential of Fumaria vaillantii extracts on three different cancer cell lines including malignant melanoma SKMEL-3, human breast adenocarcinoma MCF-7 and human myelogenous leukemia K562 as well as human gingival fibroblast (HGF) as normal cell line. Anti-proliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flowcytometry and annexin methods. Total phenolics and flavonoids were determined by Folin-Ciocalteu and aluminum chloride methods. Chloroform fraction had the lowest IC50 value at 72 h (0.1 µg/ml) in MCF-7 cells. Flowcytometry and annexin-V analysis indicated that the chloroform fraction induced necrosis in MCF-7 cells. In addition, the colorimetric methods showed that the methanolic fraction possessed the highest amount of total phenolics (33.03 ± 0.75 mg/g of dry powder) and flavonoids (10.5 ± 2.0 mg/g of dry powder).The collective data demonstrated that F. vaillantii chloroform fraction may contain effective compounds with chemo-therapeutic potential act through an apoptotic independent pathway.
