Author/Authors :
-، - نويسنده Department of Medical Microbiology, Medical Faculty, Kafkas University, Kars, Turkey Karamese, Murat , -، - نويسنده Department of General Surgery, Medical Faculty, Kafkas University, Kars, Turkey Guvendi, Bulent , -، - نويسنده Department of Histology and Embryology, Medical Faculty, Kafkas University, Kars, Turkey Aksak Karamese, Selina , -، - نويسنده Department of Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey Cinar, Irfan , -، - نويسنده Department of Physiology, Medical Faculty, Kafkas University, Kars, Turkey Can, Serpil , -، - نويسنده Department of Biochemistry, Veterinary Faculty, Ataturk University, Erzurum, Turkey Serkan Erol, Huseyin , -، - نويسنده Department of Virology, Veterinary Faculty, Ataturk University, Erzurum, Turkey Aydin, Hakan , -، - نويسنده Department of Physiology, Veterinary Faculty, Kafkas University, Kars, Turkey Gelen, Volkan , -، - نويسنده Department of Pharmacology and Toxicology, Veterinary Faculty, Ataturk University, Erzurum, Turkey Karakus, Emre
Abstract :
Objective(s): In the present study, our aim was to investigate the possible protective effects of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS)-induced hepatotoxicity by using Hep3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line Hep3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells. Materials and Methods:The Hep3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses. Results:The highest treatment dose of EGCG (400 µM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 µM) normalized all related parameters levels. Conclusion:LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways.
