Author/Authors :
Raoof، Maryam نويسنده Department of Endodontics, School of Dentistry, Kerman Medical University, Kerman, Iran , , Ebrahimnejad، Hamed نويسنده Department of Oral & Maxillofacial Radiology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran. Ebrahimnejad, Hamed , abbasnejad، mehdi نويسنده Department of Biology, Faculty of Sciences , , Amirkhosravi، Ladan نويسنده Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. Amirkhosravi, Ladan , Raoof، Ramin نويسنده Department of Biology, Faculty of Sciences , , Esmaeili-Mahani، Saeed نويسنده Department of Biology, Faculty of Sciences, , , Ramazani، Mohsen نويسنده Department of Endodontics, Mazandaran University of Medical Sciences, Sari, Iran Ramazani, Mohsen , Shokouhinejad، Noushin نويسنده , , Khoshkhounejad ، Mehrfam نويسنده Dental School, Tehran University of Medical Sciences, Tehran, Iran ,
Abstract :
Introduction: This study aimed to examine the effects of induced inflammatory tooth pain on anxiety level in adult male rats.
Methods: The mandibular incisors of 56 adult male rats were cut off and prefabricated crowns were fixed on the teeth. Formalin and capsaicin were injected intradentally to induce inflammatory tooth pain. Diazepam treated group received diazepam 30 minutes before intradental injection. The anxietyrelated behavior was evaluated with elevated plus maze test.
Results: Intradental application of chemical noxious stimuli, capsaicin and formalin, significantly affected nociceptive behaviors (P<0.001). Capsaicin (P<0.001) and formalin (P<0.01) significantly increased the anxiety levels in rats by decrease in the duration of time spent in open arm and increase in the duration of time spent in closed arm. Rats that received capsaicin made fewer open arm entries compared to the control animals (P<0.05). Capsaicin (P<0.001) and formalin (P<0.01) treated rats showed more stretch attend postures compared to the control and sham operated animals. In diazepampretreated
rats, capsaicin induced algesic effect was prevented (P<0.001).
Conclusion: Inflammatory pulpal pain has anxiogenic effect on rats, whereas diazepam premedication showed both anxiolytic and pain reducing effects.
