Author/Authors :
كوچكي ، امينه نويسنده Department of Biotechnology, School of Medicine Koochaki, Ameneh , بنده پور، مژگان نويسنده Department of Biotechnology, School of Medicine---Cellular and Molecular Research Center---Department of Biotechnology, School of Advanced Technologies in Medicine Bandehpour, Mojgan , مساحي، سام نويسنده موسسه ايرانيان ژن فناور، تهران- ايران , , صدري ، مه رو نويسنده Department of Nutrition and Health Sciences Sadri, Mahrou , كاظمي ، بهرام نويسنده Department of Biotechnology, School of Medicine----Cellular and Molecular Research Center---Department of Biotechnology, School of Advanced Technologies in Medicine Kazemi, Bahram
Abstract :
Background: Interleukin (IL)-27 is a heterodimeric cytokine belonging to IL-12 and IL-23 families, secreted by antigen presenting cells(APCs). The IL-27 is composed of 2 subunits: Epstein-Barr virus induced gene 3 (EBI3) and p28. IL-27 is an anti-inflammatory cytokine which has an inhibitory effect on Th17 population and suppress the IL-17 expression. It is suggested that IL-27 could be a potent drug candidate for treating auto immune diseases.
Materials and Methods: The EBI3 and p28 subunits of human interleukin 27 were constructed into plasmid vectors; they sub-cloned into pETDuet-1 expression vector in restriction sites of BamHI, SacI and NotI. Subsequently the induction was carried out by 1mM IPTG and the recombinant proteins were confirmed by SDS-PAGE and Western Blot analysis using anti His-tag antibody.
Results: The EBI3 and p28 subunits of human interleukin 27were cloned into plasmid vectors. The 28 and 25kDa protein bands were observed on SDS-PAGE and finally confirmed by Western blot technique.
Conclusion: In this research p28 and EBI3 proteins were produced successfully
