Author/Authors :
Jamali ، S نويسنده Assistant Professor of Mycology, Department of Plant Protection, School of Agriculture, Razi University, Kermanshah, Iran , , Gharaei ، M نويسنده MS student, Department of Plant Protectio n, School of Agriculture, Razi University, Kermanshah, Iran ,
Abstract :
Background
and Purpose:
Trichosporon
is a genus of anamorphic basidiomycetous yeast which is widely distributed
in nature and
is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and
molecular analysis of
Trichosporon
species in soil
.
Materials and Methods:
In order to isolate and identify
Trichosporon
species in soil, 30 samples were collected from
30 different locations across Iran. The isolates were identified by means of the standard methods of yeast identification.
To confirm morphological identification, genomic DNA was extracted and the hypervariable D1/D2 domain of the
large
-
subunit (LSU) ribosomal DNA (rDNA) gene was amplified by polymerase chain reaction (PCR), using primer
pair NL
-1/NL
-4, and then the sequences were analyzed
.
Results:
According to the morphological and physiological assessments, isolates were identif
ied as
T. coremiiforme
.
The isolates formed chlamydospore after one week on yeast
-malt (YM) agar medium. Using Blast program, we found
that the D1/D2 sequences of the
T. coremiiforme
isolates from Iran (accession no: KP055040 and KP055041) showed
99% homol
ogy with the
T. coremiiforme
deposited in GenBank. All the
T. coremiiforme
isolates placed in the Ovoides
cluster were well
-supported by bootstrap values
.
Conclusion:
The present study is the first attempt to survey
Trichosporon
in soil of Iran. To the bes
t of our knowledge,
this is the first investigation of
T. coremiiforme
in Iran
.
