Author/Authors :
Nazemi Salman، Bahareh نويسنده Department of Pedodontics, Dental School, Zanjan University of Medical Sciences, Zanjan, Iran; , , Vahabi، Surena نويسنده Department of Periodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran; , , Biglari، Alireza نويسنده Department of Molecular Medicine & Genetics, School of Medicine, Zanjan University of Medical Sciences , , Salavitabar، Simindokht نويسنده Bachelor of Science in Genetics, Azad Islamic University, Medical Branch, Tehran , , Hassani Doabsari، Maryam نويسنده Bachelor of Science in Genetics, Azad Islamic University, Medical Branch, Tehran ,
Abstract :
Background: Periodontal disease has a multifactorial etiology. A combination of microbial agents
and environmental, habitual, systemic, and genetic risk factors is responsible for the development of
periodontal disease. Host immune response causes the destruction of tooth?supporting structure
and eventual tooth loss. This study aimed to assess the correlation of interleukin 6 (IL?6) ?174?GC
and IL?6?572?GC gene polymorphisms with periodontal disease in an Iranian population.
Materials and Methods: This case–control analytical study was conducted on 129 subjects
presenting to the laboratory of Taleghani Hospital. Subjects underwent clinical and periodontal
examinations and divided into five groups of healthy, gingivitis and mild, moderate and severe
periodontitis. Blood samples (2 ml) were obtained. Genomic DNA was extracted manually using
the salting?out method. IL?6 sequence amplification was performed using polymerase chain reaction
with three thermal protocols. Digested products were analyzed by electrophoresis through 2%
agarose gel using Gel Red staining. Data were analyzed using Chi?square, Kruskal–Wallis, and
Mann–Whitney tests, and P < 0.05 was considered significant.
Results: The frequency of GG polymorphism at IL?6?174 and IL?6?572 genomic regions was 51.2%
and 71.3%, respectively. The frequency of IL?6?572?GG polymorphism was significantly greater than
that of IL?6?572?GC polymorphism (P < 0.001). Comparison of the mean and maximum pocket depth
and clinical attachment level, as well as bleeding on probing percentage, revealed significant differences
between the healthy controls and periodontitis patients (P < 0.001). The frequency percentages of
GC and GG polymorphisms were almost equal in the healthy, gingivitis, and periodontitis groups.
In other words, the frequency of the two polymorphisms was not significantly different between
the health and disease states (P = 0.065 for IL?6?572 and P = 0.63 for IL?6?174).
Conclusion: This study found no association between IL?6?174 and IL?6?572 gene polymorphisms
and periodontitis in the studied population.
