Author/Authors :
Saei، Azad نويسنده Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran Saei, Azad , Boghozian، Roobina نويسنده Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran Boghozian, Roobina , Mirzaei، Reza نويسنده Shiraz university , , Jamali، Arezoo نويسنده Department of Laboratory Sciences, School of Paramedicine, University of Medical Sciences, Tehran, Iran Jamali, Arezoo , Vaziri، Behrooz نويسنده Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Vaziri, Behrooz , Hadjati، Jamshid نويسنده ,
Abstract :
Fully mature dendritic cells (DCs) play pivotal role in inducing immune responses and converting naïve T lymphocytes into functional Th1 cells. We aimed to evaluate Listeria Monocytogenes-derived protein fractions to induce DC maturation and stimulating T helper (Th)1 immune responses.
In the present study, we fractionated Listeria Monocytogenes-derived proteins by adding of ammonium sulfate in a stepwise manner. DCs were also generated from C57BL/6 mice bone marrow precursor cells. Then, the effects of protein fractions on bone marrow derived DC (BMDC) maturation were evaluated. In addition, we assessed the capacity of activated DCs to induce cytokine production and proliferation of lymphocytes.
Listeria-derived protein fractions induced fully mature DCs expressing high costimulatory molecules such as CD80, CD86 and CD40. DCs that were activated by selected F3 fraction had low capacity to uptake exogenous antigens while secreted high levels of Interleukine (IL)-12. Moreover, lymphocytes cultured with activated BMDCs produced high amounts of IFN-γ and showed higher proliferation than control. Listeria derived protein fractions differently influenced DC maturation.
In conclusion, Listeria protein activated-BMDCs can be used as a cell based vaccine to induce anti-tumor immune responses.
