Author/Authors :
Karami، Mohammad نويسنده , , Ale-Nabi، Seyed Sajad نويسنده Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran , , Nosrati، Anahita نويسنده Department of pathology, Emam Hospital, Mazandaran University of Medical Science, Sari, Iran. , , Naimifar، Atefeh نويسنده Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran ,
Abstract :
The aim of present study was to investigate the potential antioxidant and lung protective activities of Sambucus ebulus (SE) against toxicity induced by Gamma irradiation. Hydro alcoholic extract of SE (20, 50 and 100 mg/kg ) was studied for its lung protective activity. . Phenol and flavonoid contents of SE were determined.
Male C57 mice were divided into 10 groups with five mice per group. Only the first and second groups (as negative control) received intraperitoneally normal saline fluid. Groups 3 to 5 received only SE extract at doses of 20 mg/kg, 50 mg/kg and 100 mg/kg intraperitoneally; three groups were repeatedly injected for 15 days as chronic group. Groups 6 to 8 received a single-dose of gamma irradiation just 2 hours before irradiation as acute group. The ninth and tenth groups (as positive control) received only gamma rays. Animal was exposed whole-body to 6 Gy gamma radiation. After irradiation, tissue sections of lung parenchyma were examined by light microscope for any histopathologic changes.
SE at doses 50 and 100mg/kg improved markedly histopathological changes induced by gamma irradiation in lung. Lung protective effect of SE could be due to attention of lipid peroxidation. Our study demonstrated that SE as a natural product has a protective effect against lung toxicity induced by gamma irradiation in animal.
