Title of article :
In Vitro Cytotoxic Effects of Celecoxib, Mefenamic Acid, Aspirin and Indometacin on Several Cells Lines
Author/Authors :
Hashemipour، Maryam Alsadat نويسنده Department of Oral Medicine, Dental School, Kerman University of Medical Science, Kerman, Iran. Hashemipour, Maryam Alsadat , Mehrabizadeh Honarmand، Hoda نويسنده Resident of Oral and Maxillofacial Surgery and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran. , , Falsafi ، Farideh نويسنده Dentist, Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran. , , Tahmasebi Arashlo، Mehrnaz نويسنده Dentist, Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran. , , Rajabalian ، Saied نويسنده Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran. , , Gandjalikhan Nassab ، Sayed Amir Hossein نويسنده Medical Student, Kerman Dental and Oral Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran. ,
Issue Information :
فصلنامه با شماره پیاپی 52 سال 2016
Abstract :
Statement of the Problem: Use of cyclooxygenase inhibitors as chemotherapy agents has attracted the attention of a large number of investigators in recent years. Given the importance of cancer therapy, only a limited number of studies have been carried out to investigate the effects of cyclooxygenase inhibitors on specific cell lines.
Purpose: This research aimed to determine the in vitro cytotoxic effects of cyclooxygenase inhibitors (COX-1 and COX-2 inhibitors) on KB, Saos-2, 1321N, U-87MG, SFBF-PI 39 cell lines.
Materials and Method: Powders of celecoxib, mefenamic acid, aspirin and indometacin were dissolved in the appropriate solvent. The viability of cell lines was carried out by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay technique. Data gathered from four separate experiments were expressed as mean±SD. Statistical significance was defined at p < 0.05 by using analysis of variance. Significant treatment mean values were subjected to post-hoc Tukey’s test.
Results: Celecoxib showed marked cytotoxic effects on KB, Saos-2, and 1321N cells, which was significant in comparison with the control group. Celecoxib was not effective in killing U-87MG cell line. Mefenamic acid exerted cytotoxic effects on KB, Saos-2, and 1321N cells, where the viability was approximately 75%. U-87MG cells were resistant to mefenamic acid. Indometacin had the highest rate of activity on U-87MG cells, which was significant in comparison with the control group. Aspirin did not exhibit any activity on these cell lines and was not effective in killing U-87MG, KB, Saos-2, and 1321N cells.
Conclusion: This research showed that celecoxib, indometacin, and mefenamic acid have the cytotoxic effects on KB, Saos-2, 1321N and U-87MG cell lines. Therefore, it appears that these drugs can be considered as anti-neoplastic agents in the experimental phase.
Journal title :
Journal of Dentistry :Shiraz University of Medical Sciences
Journal title :
Journal of Dentistry :Shiraz University of Medical Sciences