Author/Authors :
L?szlov?، K. نويسنده Slovak University of Technology, Faculty of Chemical and Food Technology, Institute of Biotechnology, Radlinského 9, 812 37 Bratislava, Slovak Republic , , Dercov?، K. نويسنده Slovak University of Technology, Faculty of Chemical and Food Technology, Institute of Biotechnology, Radlinského 9, 812 37 Bratislava, Slovak Republic , , Horv?thov?، H. نويسنده Slovak University of Technology, Faculty of Chemical and Food Technology, Institute of Biotechnology, Radlinského 9, 812 37 Bratislava, Slovak Republic , , Mur?nov?، S. نويسنده Water Research Institute, National Water Reference Laboratory, n?br. Arm. gen. L. Svobodu 5, 812 49 Bratislava, Slovak Republic , , ?karba، J. نويسنده Slovak University of Technology, Faculty of Chemical and Food Technology, Institute of Biotechnology, Radlinského 9, 812 37 Bratislava, Slovak Republic , , Dud??ov?، H. نويسنده Slovak Academy of Sciences, Institute of Chemistry, D?bravsk? cesta 1, 84538 Bratislava, Slovak Republic ,
Abstract :
The prospects of using the biostimulation and bioaugmentation treatment for the
removal of the polychlorinated biphenyl (PCBs) from contaminated sediment collected from the sewage
canal in the surroundings of a former PCB manufacturing plant in Slovakia is described. The
eleven bacterial strains isolated in our previous work from sewage Str??sky canal sediments were able
to aerobically degrade significant amounts of PCBs. Five of the bacterial isolates obtained were used
in bioaugmentation treatment individually as single strains and within the eight artificially prepared
consortia consisting of two or three strains. Bioaugmentation by a single strain was performed in
other set of experiments combined with the addition of nonionic surfactants (Triton X and Tween 80)
to increase bioavailability of PCBs and with the addition of terpenes (carvone and limonene) to
induce required enzymes.The highest biodegradation of PCBs in biostimulation treatment was obtained
using all three studied factors - addition of nitrogen, phosphorus, and oxygen to the indigenous
microorganisms naturally present in the contaminated sediment. The highest biodegradation
of PCBs in bioaugmentation experiments was obtained with the individual bacterial isolates (one
Gram-positive and one Gram-negative), and with a laboratory prepared consortium consisting of
three selected bacterial isolates. Addition of surfactant Tween 80 led to a higher biodegradation of
PCBs than that of Triton X. Bioaugmentation treatment using the addition of single bacterial isolate
and surfactant Tween 80 improved elimination of the evaluated indicator PCB congeners to the
highest extent and thus appeared to be the most perspective technique for possible field bioremediation.
