Author/Authors :
Ashkani Esfahani، Soheil نويسنده Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran , , Bagheri، Fereshteh نويسنده Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran , , Emami، Yasaman نويسنده Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, IR Iran , , Esmaeilzadeh، Elmira نويسنده Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran , , Azarpira، Negar نويسنده , , Hassanabadi، Nazila نويسنده Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran , , Keshtkar، Marzieh نويسنده International Branch, Shiraz University of Medical Sciences, Kish, IR Iran , , Farjam، Mojtaba نويسنده Department Of Pharmacology, Fasa University of Medical Sciences, Shiraz, IR Iran , , Koohi Hosseinabadi ، Omid نويسنده Stem cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. , , Noorafshan، Ali نويسنده ,
Abstract :
Acute liver damage may be followed by biochemical, behavioral, and pathological alterations, which can result in serious complications and even death. In this experimental study we determined whether coenzyme Q10 (CoQ10), a common supplementary medicine known to have protective, antioxidative, and anti-inflammatory effects in cells, has any protective effect against thioacetamide (TAA)-induced liver damage and its related neurobehavioral alterations in rats. In this experimental study forty-eight Wistar rats were divided randomly into four groups (n = 12): C1 was the control group; C2 received a single-dose of TAA (350mg/kg; intraperitoneally) without any other treatment; E1 received TAA + 5 mg/kg CoQ10 (intraperitoneally); and E2 received TAA + 10 mg/kg CoQ10. After sacrificing the rats, liver enzymes and plasma-ammonia (NH4) were measured and histopathological analyses of the livers were carried out. Elevated-plus-maze, open-field, and forced-swimming tests were also performed to investigate behavioral correlations. The serum levels of alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), and NH4 show significant increases (P < 0.05). The groups treated with CoQ10 were shown to have significantly lower clinical grade of encephalopathy (P = 0.001), higher locomotor activity (P = 0.000), and lower levels of depression (P = 0.000). Furthermore, it was also shown that CoQ10 treatment may lead to significant decreases in scores of centrilobular necrosis, apoptosis, inflammatory cell infiltration, vacuolization, and liver necrosis (P < 0.05). Overall, CoQ10 was determined to have positive effects on liver injury and its related behavioral and biochemical changes.
