Simultaneous Determination of Atenolol and Propranolol in Pharmaceutical Formulations by Third Derivative Spectrophotometric Method

در اين مطالعه، يك روش اسپكتروفتومتري مشتقي مرتبه سوم براساس روش صفر متقابل براي تعيين همزمان آتنولول و پروپرانولول توصيف شده است. عوامل موثر شامل دما و pH، بررسي و بهينه سازي شدند. تحت شرايط بهينه تجربي (درجه حرارت: oC 10 و 10-8 = pH)، منحني هاي درجه­بندي بين گستره­هاي غلظتي 8/0 تا ?g/mL 9/12 براي آتنولول و 3/0 تا ?g/mL 4/6 براي پروپرانولول خطي بودند. حد تشخيص ?g/mL 2/0 و انحراف استاندارد نسبي كمتر از 3? بدست آمد. روش پيشنهادي كاربرد رضايت بخشي براي تعيين همزمان دو دارو در فرمولاسيون هاي دارويي دارد.بازيابي قابل قبولي، در گستره 97 تا 107 درصد بدست آمد.

In this study, a third derivative spectrophotometric method based on zero-crossing technique is developed for the simultaneous determination of atenolol and propranolol. Effective parameters including temperature and pH were investigated and optimized. Under optimum experimental conditions (temperature: 10 oC and pH: 8-10), calibration curves were linear between concentration ranges of 0.8 to 12.9 ?g mL-1 of atenolol and 0.3 to 6.4 ?g mL-1 of propranolol. The limit of detection was 0.2 ?g mL-1 and relative standard deviation was lower than 3%. The proposed method was applied for the simultaneous determination of both drugs in the pharmaceutical formulations. Acceptable recoveries of the analytes, ranging from 97 to 107%, were obtained.