Author/Authors :
Asgharzadeh، Mohammad نويسنده , , Ghorghanlu، Sajjad نويسنده Biotechnology Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Ghorghanlu, Sajjad , Rashedi، Jalil نويسنده Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. , , Mahdavi Poor ، Behroz نويسنده Department of Laboratory Sciences, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran , , Khaki-Khatibi، Fatemeh نويسنده Khaki-Khatibi, Fatemeh , Moaddab، Seyyed Reza نويسنده Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. , , SAMADI KAFIL، Hossein نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran SAMADI KAFIL, Hossein , Pourostadi، Mahya نويسنده Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran ,
Abstract :
Promoter polymorphism of cytokine genes may lead to inter-individual differences in cytokine levels, therefore, polymorphisms may associate with susceptibility to infectious diseases. In this study, we investigated a possible association between interleukin-10 (IL-10) -1082A⁄G (rs1800896) and interferon (IFN)-gamma +874T/A (rs2430561) promoter polymorphisms and tuberculosis (TB) in the Azeri population of Iran. IL-10 -1082G/A and IFN-gamma +874T/A single nucleotide polymorphisms (SNPs) were genotyped by amplification refractory mutation system (ARMS)-PCR in 200 healthy controls and 124 tuberculosis patients. IL-10 -1082 A allele was more frequent in the control group than in the patient group (p=0.001, odds ratio [OR]=2.183). On the other hand, the AA genotype was significantly more frequent in the control group (p=0.0001). The frequency of IFN-gamma +874 T allele was significantly higher in the controls (p=0.013, OR=1.56). There was no significant association between IFN-gamma +874 T/A genotypes and susceptibility to tuberculosis (p=0.078), but TT genotype was more frequent in the control group. Our findings suggest that interleukin-10 -1082G/A polymorphism may play an important role in susceptibility to tuberculosis in our population. On the other hand, the +874T allele, which has been suggested to be associated with high IFN-gamma levels, was significantly higher in the controls and TT genotype was also more frequent in the control group. Thus, +874 T allele may be associated with resistance to tuberculosis in this Azeri population of Iran.
