Apoptosis of Human Lymphocytes after Exposure to Hydatid Fluid
Mokhtari Amirmajdi، M نويسنده Department of Mycoparasitology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Mokhtari Amirmajdi, M , Sankian، M نويسنده Immunobiochemistry Laboratory, Immunology Research Center, Bu-Ali research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Sankian, M , Eftekharzadeh Mashhadi، I نويسنده Department of Mycoparasitology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Eftekharzadeh Mashhadi, I , Varasteh، A نويسنده Immunobiochemistry Laboratory, Immunology Research Center, Bu-Ali research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Varasteh, A , Vahedi، F نويسنده Immunobiochemistry Laboratory, Immunology Research Center, Bu-Ali research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Vahedi, F , Sadrizadeh، A نويسنده Department of Thoracic surgery, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Sadrizadeh, A , Spotin، A نويسنده Department of Mycoparasitology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Spotin, A
Background: Modulation of the immune response is an important strategy by which establishment and growth of hydatid cyst in the internal organs of human is warranted. Induction of apoptosis in the lymphocytes might be a considerable component. This study was designed to evaluate apoptotic impact of hydatid fluid (HF) on human lymphocytes.
Methods: Human lymphocytes were treated with hydatid fluid. After 6 hours of exposure, caspase-3 activity, the central enzyme of apoptosis cascade, was measured by fluorometric assay in the HF-treated lymphocytes and control cells. In addition, the expression of Bax (a pro-apoptotic protein) and Bcl-2 (an anti-apoptotic protein) mRNA was assessed by RT-PCR after 12 hours of exposure.
Results: Both the ratio of Bax/Bcl-2 mRNA expression and Caspase-3 activity were higher in the HF-treated lymphocytes relative to the control group.
Conclusion: Apoptosis could be as a possible mechanism by which Echinococcus granulosus overwhelms host defenses.