Author/Authors :
Rezaeyan، A نويسنده Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , , Fardid، R نويسنده Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , , Haddadi ، G H نويسنده 1Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , , Takhshid، M A نويسنده Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , , Hosseinzadeh، M نويسنده Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , , Najaf، M نويسنده Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran , , Salajegheh، A نويسنده Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran ,
Abstract :
Background: Oxidative stress plays an important role in the pathogenesis and
progression of ?-irradiation-induced cellular damage, Lung is a radiosensitive organ
and its damage is a dose-limiting factor in radiotherapy. The administration of dietary
antioxidants has been suggested to protect against the succeeding tissue damage. The
present study aimed to evaluate the radioprotective effcacy of Hesperidin (HES)
against ?-irradiation-induced tissue damage in the lung of male rats.
Materials and Methods: Thirty two rats were divided into four groups. Rats
in Group 1 received PBS and underwent sham irradiation. Rats in Group 2 received
HES and underwent sham irradiation. Rats in Group 3 received PBS and underwent
?-irradiation. Rats in Group 4 received HES and underwent ?-irradiation. These rats
were exposed to ?-radiation 18 Gy using a single fraction cobalt-60 unit, and were
administered HES (100 mg/kg/d, b.w, orally) for 7 days prior to irradiation. Rats in
each group were sacrifced 24 hours after radiotherapy (RT) for the determination
of superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA) and
histopathological evaluations.
Results: Compared to group 1, the level of SOD and GSH signifcantly decreased
and MDA level signifcantly increased in group 3 at 24 h following irradiation,
(p=0.001, p < 0.001, p=0.001), respectively. A statistically signifcant difference in all
parameters was observed for rats in group 4 as compared to group 3 (p < 0.05). Histopathological results 24 hours after RT showed that radiation has increased in?ammation, lymphocyte, macrophage and neutrophil compared to group 1 ( p < 0.0125). Oral
administration of HES before RT signifcantly decreased macrophage and neutrophil
when compared to group 3 (p < 0.0125), but partly there was in?ammation and lymphocyte that indicated there was no signifcant difference when compared to group 3
(p > 0.0125).
Conclusion: Oral administration of HES was found to offer protection against
?-irradiation- induced pulmonary damage and oxidative stress in rats, probably by
exerting a protective effect against in?ammatory disorders via its free radical scavenging and membrane stabilizing ability.
