Author/Authors :
Ebrahimi، S. نويسنده Faculty of Agriculture,Department of Plant Protection,University of Zabol,Zabol,Iran , , Sabbagh، S. K. نويسنده Institute of Plant Biotechnology,Department of Plant Protection,University of Zabol,Zabol,Iran , , Aminaei، M. نويسنده Research Center of Agriculture and Natural Resources of Kerman Province,Kerman,Iran , , Khosravimoghadam، F. نويسنده Faculty of Agriculture,Department of Plant Protection,University of Zabol,Zabol,Iran , , Rostami، F. نويسنده Faculty of Agriculture,Department of Plant Protection,University of Zabol,Zabol,Iran ,
Abstract :
Die-back disease caused by Paecilomyces variotti is one of the most destructive diseases of pistachio in Iran. In this study, genetic diversity of Paecilomyces variotti isolates was investigated with SSR marker, using six primers pairs. Sling was done from pistachio gardens in different geographical zones of Kerman province during summer 2013. Various fungal isolates were isolated from infected twigs on PDA. Based on the morphological criteria, seventy isolates were identified as P. variotii. These identifications were confirmed by sequencing data analysis. Twenty isolates were selected for the genetic diversity assay. A total of 22 alleles was identified with an average of two alleles per each primer pair. The mean of Neis coefficient among isolates was 1.53. The average of Polymorphic Information Contents (PIC) for the SSR markers in P. variotti was 0.68. UPGMA Clustering grouped all the tested isolates into eight groups with a high genetic diversity. Based on these results, we can conclude that SSR markers are suitable for illustrating levels of genetic diversity within the isolates, but it is not adequate for demonstrating the relationship between a selected set of isolates and their related geographical regions.
Keywords :
sequencing , Molecular marker , Pathogen , pistachio
