Author/Authors :
Dinarvand، Amin نويسنده Science and Research Branch of Islamic Azad University, Tehran, Iran. Dinarvand, Amin , Goodarzi، Ali نويسنده Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran. Goodarzi, Ali , Vousooghi، Nasim نويسنده Organization Vousooghi, Nasim , Hashemi، Mehrdad نويسنده , , Dinarvand، Rasoul نويسنده Department of Pharmacoeconomics and Pharmaceutical Administration, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Dinarvand, Rasoul , Ostadzadeh، Fahimeh نويسنده Science and Research Branch of Islamic Azad University, Tehran, Iran. Ostadzadeh, Fahimeh , Khoshzaban، Ahad نويسنده Department of Oral and Maxillofacial Pathology, School of Dentistry, Shahid Beheshti Medical Science University, Tehran, Iran , , Zarrindast، Mohammad Reza نويسنده ,
Abstract :
Introduction: Association between single-nucleotide polymorphisms (SNPs) in mu opioid receptor gene and drug addiction has been shown in various studies. Here, we have evaluated the existence of polymorphisms in exon 3 of this gene in Iranian population and investigated the possible association between these mutations and opioid addiction.
Methods: 79 opioid-dependent subjects (55 males, 24 females) and 134 non-addict or control individuals (74 males, 60 females) participated in the study. Genomic DNA was extracted from volunteers’ peripheral blood and exon 3 of the mu opioid receptor gene was amplified by polymerase chain reaction (PCR) whose products were then sequenced.
Results: Three different heterozygote polymorphisms were observed in 3 male individuals: 759T>C and 877G>A mutations were found in 2 control volunteers and 1043G>C substitution was observed in an opioid-addicted subject. Association between genotype and opioid addiction for each mutation was not statistically significant.
Discussion: It seems that the sample size used in our study is not enough to confirm or reject any association between 759T>C, 877G>A and 1043G>C substitutions in exon 3 of the mu opioid receptor gene and opioid addiction susceptibility in Iranian population.
