Author/Authors :
Solhi، H. نويسنده Associated Professor of Forensic Medicine & Toxicology, School of medicine, Arak University of Medical Sciences, Arak, Iran. , , Kazemifar، AM. نويسنده Assistant professor of Forensic Medicine & Toxicology, School of medicine, Qazvin University of Medical Sciences, Qazvin, Iran. , , Khoddami Vishteh، HR نويسنده General practitioner, Tehran, Iran. , , Azizabadi Farahani، M نويسنده General practitioner, Tehran, Iran. , , Badakhshan، D. نويسنده M.Sc. of Chemistry, Arak Legal Medicine & Toxicology Laboratory, Arak, Iran. ,
Abstract :
The present study aims to present a new method to trace narcotics in the solution extracted from dried urine
stain left on cotton fabric. Narcotic substance was traced in the 50 samples through two methods. In the first
method, samples were directly examined through Thin Layer Chromatography (TLC). In the second method, a
piece of cotton fabric was soaked in a beaker filled with urine sample. After full absorption, the piece of fabric
was left to dry. Then it was soaked in distilled water and shaken so that the stain deposits would dissolve into
water. Finally, the solution was extracted from the wet fabric by centrifugal spin and admixed with an equal
amount of distilled water. A TLC test was run afterward. The TLC run on the main samples produced the
following results: morphine, codeine and other opium alkaloids were detected in 38 samples; in 7 samples only
codeine was found. Five samples produced no especial stains. The TLC performed on the solution tapped from
the cotton piece of fabric produced similar results except for the lower density of stain colors. The results show
that narcotics may be detected using dried urine stains on cotton fabric dissolved in distilled water.
