Polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene associated with growth traits in Cyprinus carpio

The genetic polymorphism of insulin-like growth factor binding protein (IGFBP-3) and its association with the growth traits namely condition factor, body weight, and total length were investigated in common carp Cyprinus carpio using PCR- single strand conformation polymorphism (SSCP) technique. Caudal fin clips from 120 C. carpio, aging 5 months, were used in this study and a 330 bp DNA fragment was amplified from 5-UTR of carp IGFBP-3. SSCP genotyping yielded seven different banding patterns namely A, B, C, D, E, F, and G with frequencies of 14.16, 12.5, 21.67, 9.17, 11.67, 10.0, and 20.84, respectively. The obtained results showed a relatively high polymorphic behavior of 5-UTR regulatory region of studied locus; therefore it shows the importance of this region in regulation of transcription of IGFBP-3 gene in carp. Marker-trait association showed significant relationships between the detected genotypes and condition factor (CF). IGFBP-3 gene polymorphism in joint with CF signifies relatively high genetic diversity and significant potential for marker-assisted selection in this species.