Molecular Detection of Ureaplasma urealyticum from Prostate Tissues using PCR-RFLP, Tehran, Iran

Background: In most cases, prostatitis can be caused by a bacterial agent such as Ureaplasma urealyticum. Considering to the cumbersome of the culture method for the detection of Ureaplasma species in clinical sles such as prostate PCR method that is faster and more appropriate than the cultivation methods, can be utilized for the detection of U. urealyticum and U. parvum. PCRRFLP method can differentiate both biovars and assist in studies of the clinical diagnosis, epidemiology and pathology of this species in human. The aim of this study was to molecular detection of U. urealyticumin in prostate tissue sles based on PCR RFLP. Methods:Two hundred prostate tissue sles were collected from patient suffering from prostatitis. The PCR assay was used to lify a 559 bp fragment of 16S23SRNA interspace region of Ureaplasma. After sequencing, PCR products from positive sles were digested with TaqI restriction enzyme. Results: Seven cases (3.5%) out of 200 prostate tissue sles were positive for U. urealyticum. Results of PCR products sequencing demonstrated that all isolates were U. parvum biovar. PCRRFLP results shown that there was not any differentiation in pattern of enzymatic digestion, in addition, all isolates were U. parvum, serovar 3. Conclusion: U. urealyticum can be one of the causing agents of prostatitis. Using PCRRFLP with specific primer and restriction enzyme is a rapid and costeffect method for detection and differentiation of Ureaplasma from clinical samples.