Investigation of Enzyme Immobilization Effects on its Characteristics

Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve their characteristics. Methods; In this study silica coated magnetite nanoparticles were Functionalized with GPTSM as a linker, then immobilization reaction performed by using  various amounts of lipase B from Candida Antarctica (CALB), for the next step immobilization effects on thermal stability and optimum pH were investigated in comparison with free CALB. Results; Results illustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55°C while free CALB loss its activity at the same condition. Conclusion; Immobilization of CALB on Fe3O4@SiO2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free CALB.