Author/Authors :
Aghazadeh، Mohammad نويسنده Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran. , , SAMADI KAFIL، Hossein نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran SAMADI KAFIL, Hossein , Ghotaslou، Reza نويسنده Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. , , Asgharzadeh، Mohammad نويسنده , , Moghadami، Maryam نويسنده Department of Knowledge and Information Science, Tarbiat
Modares University, Tehran, IR Iran , , Akhi، Mohammad Taghi نويسنده Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. , , Hojabri، Zoya نويسنده Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran. , , Naghili، Behrouz نويسنده Research Center of Infectious Diseases and Tropical Medicine, Tabriz University of Medical Science, Tabriz, Iran . , , Najafi، Khadijeh نويسنده Drug Applied Research Center, Tabriz University of Medical
Sciences, Tabriz, Iran , , Azimi، Somayeh نويسنده Infectious and Tropical Disease Research Center, Tabriz
University of Medical Sciences, Tabriz, IR Iran , , Shokrian، Saeed نويسنده Infectious and Tropical Disease Research Center, Tabriz
University of Medical Sciences, Tabriz, IR Iran ,
Abstract :
Pseudomonas aeruginosa is main bacterial pathogen accountable for
nosocomial infections. Furthermore, it could potentially become
resistant to β-lactams, aminoglycosides and fluoroquinolones
antibiotics. The purpose of this study was to determine the antibiotic
susceptibility pattern and genotyping of P. aeruginosa in hospitals of
Tabriz (Iran) and investigate the prevalence of OXA producer isolates.
Overall, 151 non-replicated isolates of P. aeruginosa were collected
from October 2013 until September 2014. Antibiotic susceptibility
pattern was determined by disk diffusion (Kirby Bauer) method, according
to the clinical laboratory standards institute (CLSI) guideline. Genes
encoding OXA (Ambler class D) β-lactamase were detected by PCR for all
isolates. Polymerase chain reaction with Enterobacterial repetitive
intergenic consensus-PCR (ERIC-PCR) primers was used to establish the
clonal relationship between the different isolates. The frequencies of
resistance to antibiotics were as follows: gentamicin: 68%, ceftazidime:
67%, piperacillin: 66%, cefepime: 64%, ciprofloxacin: 62%, tobramycin:
61%, amikacin: 60%, imipenem: 52%, gatifloxacin: 28%, polymyxin B: 2 %
and colistin: 2%. The OXA group I genes was identified in 82 (56%), the
OXA group II gene in 26 (18%), OXA group III in 14 (9%), OXA-1 in 22
(15%) and OXA-4 in 3 (2%) isolates. The ERIC-PCR indicated high genetic
diversity among P. aeruginosa isolates. The high prevalence of OXA
β-lactamase and high genetic diversity of P. aeruginosa indicated that
the resistance of P. aeruginosa might be expanding in our studied
hospitals.
