Author/Authors :
Praveen، US نويسنده Regional Forensic Science Laboratory, Gulbarga, India , , Gowtham، MD نويسنده Regional Forensic Science Laboratory, Belgaum, India , , Yogaraje-Gowda ، CV نويسنده Department of Biochemistry, Bangalore Medical College, Bangalore, India , , Nayak ، VG نويسنده Forensic Science Laboratory, Bangalore, India , , Mohan ، BM نويسنده Forensic Science Laboratory, Bangalore, India ,
Abstract :
Background: Nerium oleander is an evergreen shrub of
Apocynaceae family cultivated worldwide as an ornamental
plant. All parts of the plant are toxic and contain a mixture of
very toxic cardiac glycosides of cardenolides. A number of
techniques were used to determine the cardenolides of
N.oleander in various biological matrices. A survey of
literature has revealed that the use of high-performance thinlayer
chromatography (HPTLC) for the detection of oleander
glycosides is very scanty.
Method: A simple and rapid HPTLC method for separation
and identification of cardenolides of N.oleander is reported.
The cardenolides present in the aerial parts of the plant and
residues available in the autopsy samples sent in cases of
poisoning; were extracted with chloroform by using
accelerated solvent extractor (ASE).
Results: Separation of cardenolides was achieved on precoated
silica gel 60F254 HPTLC plates with chloroform-acetone-acetic
acid 8.5:1:0.5 (v/v) as mobile phase and densitometric analysis
was carried out at 275 nm. A comprehensive study for the
separation and detection of cardenolides in general and
oleandrin in particular were studied by new mobile phases and
spray reagents. The 1H-NMR spectra were recorded for the
separated components and the component corresponding to
oleandrin was identified.
Conclusion: The method has specific advantages that it is
simple, rapid and has higher resolution of separation achieved
so as to be free from interferences from the plant and forensic
matrices.
