Author/Authors :
Pooladi، M. نويسنده School of Basic Sciences,Department of Biology,Islamic Azad University (IAU), Science and Research Branch,Tehran,Iran , , Rezaei-Tavirani، M. نويسنده Proteomics Research Center, Faculty of Paramedical Sciences,Shahid Beheshti University of Medical Sciences,Tehran,Iran , , Hashemi، M. نويسنده Department Of Molecular Genetics,Islamic Azad University Tehran, Tehran Medical Branch,Tehran,Iran , , Hesami-Tackallou، S. نويسنده Department of Biology,Islamic Azad University, Garmsar Branch,Garmsar,Iran , , Khaghani Razi Abad، S. نويسنده School of Basic Sciences,Department of Biology,Islamic Azad University, Science and Research Branch,Tehran,Iran , , Firozi Dalvand، L. نويسنده School of Basic Sciences,Department of Biology,Islamic Azad University, Science and Research Branch,Tehran,Iran , , Alaei، A. نويسنده School of Basic Sciences,Department of Biology,Islamic Azad University, Science and Research Branch,Tehran,Iran ,
Abstract :
EGFR is a key molecule in cancer cells. EGFR signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. We extracted proteins from astrocytoma (III and IV) oligodendroglioma(IV) tumors and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometry method. In this study, we separated proteins by the twodimensional gel (2DG) electrophoresis method, and the spots were analyzed and compared using statistical data and MatrixAssisted Laser Desorption/Ionization TimeOfFlight Mass Spectrometry (MALDITOFTOF MS). EGFR position showed in the diagram cluster for oligodendroglioma tumors (ttest and oneway ANOVA P lt 0.05). EGFR proteins were definitely with isoelectric pH, molecular weight and protein databank detected which has an upregulation. Proteomics analysis revealed an unexpected alteration in the expression of certain protein networks in EGFR gliomas. The EGFR dependent signaling pathways are most frequently reported in high grade oligodendroglioma and affect all histological closes. Thus EGFR could be a candidate biomarker in glioma tumors.
