Author/Authors :
Khodavaisy، Sadegh نويسنده Department of Medical Parasitology and Mycology, Kurdistan University of Medical Sciences, Sanandaj, Iran. Khodavaisy, Sadegh , Rezaie، Sassan نويسنده , , Noorbakhsh، Fatemeh نويسنده , , Baghdadi، Elham نويسنده Department of Microbiology, Faculty of Science, Islamic
Azad University, Varamin-Pishva, IR Iran , , Sharifynia، Somayeh نويسنده Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Sharifynia, Somayeh , Aala، Farzad نويسنده Department of Medical Mycology and Parasitology, Kurdistan
University of Medical Sciences, Sanandaj, IR Iran ,
Abstract :
Aflatoxins are highly toxic secondary metabolites mainly produced
by Aspergillus parasiticus. This species can contaminate a wide range of
agricultural commodities, including cereals, peanuts, and crops in the
field. In recent years, research on medicinal herbs, such as Pistacia
atlantica subsp. kurdica, have led to reduced microbial growth, and
these herbs also have a particular effect on the production of
aflatoxins as carcinogenic compounds. In this study, we to examine P.
atlantica subsp. kurdica as a natural compound used to inhibit the
growth of A. parasiticus and to act as an anti-mycotoxin. In vitro
antifungal susceptibility testing of P. atlantica subsp. kurdica for A.
parasiticus was performed according to CLSI document M38-A2. The rate of
aflatoxin production was determined using the HPLC technique after
exposure to different concentrations (62.5 - 125 mg/mL) of the gum. The
changes in expression levels of the aflR gene were analyzed with a
quantitative real-time PCR assay. The results showed that P. atlantica
subsp. kurdica can inhibit A. parasiticus growth at a concentration of
125 mg/mL. HPLC results revealed a significant decrease in aflatoxin
production with 125 mg/mL of P. atlantica subsp. kurdica, and AFL-B1
production was entirely inhibited. Based on quantitative real-time PCR
results, the rate of aflR gene expression was significantly decreased
after treatment with P. atlantica subsp. kurdica. Pistacia atlantica
subsp. kurdica has anti-toxic properties in addition to an inhibitory
effect on A. parasiticus growth, and is able to decrease aflatoxin
production effectively in a dose-dependent manner. Therefore, this
herbal extract maybe considered a potential anti-mycotoxin agent in
medicine or industrial agriculture.
