Expression Level of the PPP2R5C Gene in Patients With Acute Myeloid Leukemia and Chronic Myeloid Leukemia

Background One of the most important B-subunits of protein phosphatase 2A (PP2A) is PPP2R5C, which is involved in both the suppression and promotion of cell growth. Objectives With regard to this vital role of PPP2R5C, we will examine whether alteration in expression of some proteins is involved in signal transduction, for example the B-subunit of protein phosphatases can be observed in patients with acute myeloid leukemia (AML) and chronic myeloid leukemia (CML). Materials and Methods We recruited 50 patients with AML and 31 patients with CML, together with 23 individuals in the control group. The expression levels of PPP2R5C and the reference gene B2-MG were determined by SYBR green I real-time PCR. The 2^(−ΔΔCT) method was used to present the data of the genes of interest relative to an internal control gene. Results In this study, significantly higher expression of PPP2R5C was found in the AML (4.13 ± 4.85) compared with healthy individuals. The expression level of PPP2R5C significantly decreased (0.469 ± 0.34) in CML patients compared with healthy individuals. Conclusions With regard to the critical role of PPP2R5C B-subunit on regulation of PP2A, the change in the expression pattern of PPP2R5C may be a critical finding in AML and CML patients, because it could be a cause of abnormal cell proliferation, differentiation, and transformation in these two groups of patients.