The Effects of Influencing Factors on γ-carboxylation and Expression of Recombinant Vitamin K Dependent Coagulation Factors

Vitamin K-dependent coagulation factors (VKD) have an important role in the treatment of bleeding disorders. Any disturbance in production, processing, or post-translational modifications (PTM) of these factors inhibitsthe production of functional coagulation proteins. Among the required PTMs, γ-carboxylation is a unique PTM which presents a major challenge in obtaining high expression of fully functional recombinant proteins. Studies have shown that the rate of γ-carboxylation directly relates to the amount of produced functional proteins and that it can be influenced by several factors. Here, we investigate the effects of influencing factors on γ-carboxylation and expression of VKD recombinant proteins in expression systems.