Author/Authors :
Gholami، Mohammadreza نويسنده , , Saki، Ghasem نويسنده Department of Anatomy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , , Hemadi، Masoud نويسنده Associate Professor, Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , , Khodadadi، Ali نويسنده Department of Immunology, School of Medicine , Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , , Mohammadiasl، Javad نويسنده Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ,
Abstract :
Freezing of the immature testis tissue and then its transplantation
can be considered a major step in fertility preservation for young boys
with cancer. The objective of this study was to investigate the
antioxidant effects of melatonin on histological structure of
seminiferous tubules that were vitrified-thawed with different
cryoprotectant media. Male mice were randomly assigned to one of 10
experimental groups. Testes of 6-day-old BALB/c male mice pups were
extracted and vitrified-thawed with different vitrification media with
melatonin and without melatonin. Then, their testes were stained with
haematoxylin-eosin. The result showed that supplementation of glycerol
based media with melatonin has reduced injury to histological structure
of seminiferous tubules in the 6-day-old male BALB/c mice pups testes (P
= 0.004). It seem that melatonin due to its antioxidant properties, if
added to glycerol based media, reduces the harmful effects of
freezing-thawing process, and protects seminiferous tubules histological
structure from oxidative injury.
