Author/Authors :
Delshad، Navid نويسنده Biotechnology Research Center, Mashhad University of
Medical Sciences, Mashhad, IR Iran , , Ghayour-Mobarhan، Majid نويسنده MD, PhD, Cardiovascular Research Center, Avicenna (Bu-Ali) Research Institute, Mashhad University of Medical Science, Mashhad , , Mirzaei Ghazikalayeh، Hamed نويسنده Department of Microbiology and Immunology, Kashan University of Medical Sciences, Kashan, IR Iran , , Razavi-Azarkhiavi، Kamal نويسنده Department of Pharmacodynamics and Toxicology, School of
Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR
Iran , , Moohebati، Mohsen نويسنده Mashhad University of Medical Sciences , , Hassany، Mitra نويسنده Biotechnology Research Center, Mashhad University of
Medical Sciences, Mashhad, IR Iran , , Kasaian، Jamal نويسنده Biotechnology Research Center, Mashhad University of
Medical Sciences, Mashhad, IR Iran , , Etemadzadeh، Mohammad Reza نويسنده Cardiovascular Research Center, Avicenna Research
institute, Mashhad University of Medical Sciences, Mashhad, IR
Iran , , Alavi، Maryam Sadat نويسنده Cardiovascular Research Center, Avicenna Research
institute, Mashhad University of Medical Sciences, Mashhad, IR
Iran , , Behravan، Javad نويسنده ,
Abstract :
There are very limited data for Iranian populations on the
predisposing genetic factors for acute coronary syndrome (ACS). The
objective of the present study was to investigate the association of the
angiotensin II type 1 receptor (AT1R) gene polymorphism and ACS in an
Iranian population. This cross-sectional study was conducted among 263
subjects (97 men and 166 women). Patients (n = 128) aged 30 - 80 years
with chest pain were recruited from the emergency department of Ghaem
Hospital (Mashhad, Iran). A 12-lead electrocardiograph plus creatine
kinase MB (CK-MB) levels were used as the basis for the diagnosis of
myocardial ischemia. The control group was selected from age-matched
healthy subjects (n = 135). Non-enzymatic kits were used for extraction
of DNA from blood samples. Polymerase chain reaction (PCR) was performed
to amplify the DNA fragments. For restriction fragment length
polymorphism (RFLP) determination, the DdeI enzyme was used to digest
the amplified DNA fragments. Statistical analyses were performed using
SPSS version 13.0. There was no statistical difference in the genotype
frequency of patients and healthy subjects with regard to age and gender
(P > 0.05). The AT1R A1166C polymorphism appeared not to be
associated with the presence of ACS in the population studied.
