Author/Authors :
Mohmed Khair، Osama Mohamed نويسنده Department of Virology, Central Laboratory, Ministry of
Higher Education, Khartoum, Sudan , , Enan، Khalid Abd Allah نويسنده Department of Immunology, Central Laboratory, Ministry of
Higher Education, Khartoum, Sudan , , Hussien، Mohammed Osman نويسنده Department of Virology, Central Laboratory, Ministry of
Higher Education, Khartoum, Sudan , , Mohammed، Abdalhafeez Abd Alazeem نويسنده Department of Virology, Central Laboratory, Ministry of
Higher Education, Khartoum, Sudan , , Bozdayi، Mithat A. نويسنده Hepatology Institute University of Ankara, Ankara,
Turkey , , Karatayli، Ersin نويسنده Hepatology Institute University of Ankara, Ankara,
Turkey , , Elhussein، Abd Alrheem Mohamed نويسنده Institute of veterinary research (IVR) animal resource
research corporation (ARRC), Khartoum, Sudan , , Elkhider، Isam Mohammed نويسنده Department of Microbiology and Parasitology, Faculty of
Medicine, University of Khartoum, Khartoum, Sudan , , Yurdaydin، Cihan نويسنده Hepatology Institute University of Ankara, Ankara,
Turkey ,
Abstract :
The hepatitis delta virus (HDV) was first discovered by Rizzetto in
1977 in a patient with chronic hepatitis B virus (HBV) infection. In
1980, it was shown that HDV was an infectious agent responsible for
exacerbation of liver disease in patients with hepatitis. The aim of
this study was to determine the seroreactivity of, and to molecularly
detect, HDV among hemodialysis patients and blood donors in Khartoum
state, Sudan, during the period January 2012 to July 2013. Two hundred
and seventy-six plasma samples were collected from hepatitis B surface
antigen-positive (HBsAg) patients in Khartoum state. Of these patients,
98 (64 males and 34 females) were hemodialysis patients, and 178 (178
males) were from blood donors. The sera were screened for HBsAg to
confirm positivity for HBV, HDV IgG and IgM antibodies using commercial
ELISA and HDV RNA using semi-nested RT-PCR. The results showed that
16.0% (16/100) had antibodies against HDV IgG, while only 13.0% (13/100)
had HDV IgM antibodies among hemodialysis patients, while 4.5% (8/178)
had antibodies against IgG, and 2.8% (5/178) had IgM antibodies among
blood donors. HDV RNA was detected in 13.2% (13/98) of hemodialysis
patients; of these 12.2% (7) were male, and 17.6% (6) were female. The
high prevalence of HDV in Khartoum State was documented through
detection of HDV-specific antibodies and viral RNA. Further studies
using various diagnostic methods should be considered to determine the
incidence and the common genotype of HDV disease at the country
level.
