Title of article
Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold
Author/Authors
-، - نويسنده Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran|Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Sahab Negah, Sajad , -، - نويسنده Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Aligholi, Hadi , -، - نويسنده Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran Khaksar, Zabihollah , -، - نويسنده Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran Kazemi, Hadi , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Modarres Mousavi, Sayed Mostafa , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran|Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Safahani, Maryam , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Barati Dowom, Parastoo , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran|Epilepsy Research Center, Westf?lische Wilhelms-Universit?t Münster, Germany|Department of Neurology and Department of Neurosurgery, Westf?lische Wilhelms-U Gorji, Ali
Issue Information
ماهنامه با شماره پیاپی 0 سال 2016
Pages
8
From page
1271
To page
1278
Abstract
-
Abstract
Objective(s): In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs). Materials and Methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix. Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment. Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.
Journal title
Iranian Journal of Basic Medical Sciences
Serial Year
2016
Journal title
Iranian Journal of Basic Medical Sciences
Record number
2400161
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