Author/Authors :
Yolmeh، Mahmoud نويسنده 1Faculty of Food Sciences and Technology, Gorgan University of Agriculture Sciences and Natural Resource, Gorgan, IR Iran Yolmeh, Mahmoud , Hamedi، Hasan نويسنده Dept. of Radiation Oncology, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran , , Khomeiri، Morteza نويسنده Department of Food Science, Technology and Engineering, Faculty of Agricultural Engineering, Tehran University, Karaj, Iran ,
Abstract :
Background Nowadays hazards of synthetic additives and
preservatives have been identified, so researchers are looking to a
natural and safe alternative for them. The aim of this study was to
evaluate antimicrobial effect of carotenoids of Rhodotorula
glutinis on the some pathogenic bacteria and fungi. Methods
This experimental study was done in Gorgan University of Agriculture and
Natural Resources. After cultivating R. glutinis in
50 mL YPG broth at 30°C for overnight, cells were harvested by
centrifugation at 10,000 rpm for 10 minutes and were washed three times
with distilled water. Cells were ruptured 3 times with 12 mL of acetone
and broken using homogenizer. Then the suspension was centrifuged and
the supernatant collected. The supernatant (contain pigments) was
powdered using freeze-dryer. Antimicrobial activity was evaluated by
disc diffusion method and the minimum inhibitory concentration (MIC) and
minimum bactericidal concentration (MBC) was determined by using the
agar dilution method. Results Giving the results, carotenoids of
R. glutinis was effective on the growth of all the
tested bacteria, so that Bacillus cereus and
Salmonella enteritidis were the lowest and highest
sensitivity to this pigment, respectively. The highest MIC and MBC among
the tested bacteria were observed for S. enteritidis
and Escherichia coli, respectively; whereas MBC was
not observed for S. enteritidis at concentrations of
the tested pigment. Conclusions Gram-positive bacteria were more
sensitive than Gram-negative bacteria against the antimicrobial activity
of pigments of R. glutinis. According to the results,
pigments of R. glutinis can be used as an inhibitor
of bacterial growth.
