Author/Authors :
Yazdian–Robati، Rezvan نويسنده School of Pharmacy,Department of Pharmaceutical Biotechnology,Mashhad University of Medical Sciences,Mashhad,Iran , , Ahmadi، Homa نويسنده School of Pharmacy,Department of Pharmacodynamics and Toxicology,Mashhad University of Medical Sciences,Mashhad,Iran , , Matbou Riahi، Maryam نويسنده School of Medicine,Department of Medical Biotechnology,Mashhad University of Medical Sciences,Mashhad,Iran , , Lari، Parisa نويسنده School of Pharmacy,Department of Pharmacodynamics and Toxicology,Mashhad University of Medical Sciences,Mashhad,Iran , , Aledavood، Amir نويسنده Faculty of Medicine, Cancer Research Center,Department of Radiation oncology,Mashhad University of Medical Sciences,Mashhad,Iran , , Rashedinia، Marzieh نويسنده School of Pharmacy,Department of Pharmacology and Toxicology,Shiraz University of Medical Sciences,Shiraz,Iran , , Abnous، Khalil نويسنده Pharmaceutical Research Center, School of Pharmacy,Mashhad University of Medical Sciences,Mashhad,Iran , , Ramezani، Mohammad نويسنده School of Pharmacy, Nanotechnology Research Center,Department of Biotechnology,Mashhad University of Medical Sciences,Mashhad,Iran ,
Abstract :
Objective(s): Ranking as the sixth commonest cancer, esophageal squamous cell carcinoma (ESCC) represents one of the leading causes of cancer death worldwide. One of the main reasons for the low survival of patients with esophageal cancer is its late diagnosis. Materials and Methods: We used proteomics approach to analyze ESCC tissues with the aim of a better understanding of the malignant mechanism and searching candidate protein biomarkers for early diagnosis of esophageal cancer. The differential protein expression between cancerous and normal esophageal tissues was investigated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Then proteins were identified by matrix-assisted laser desorption/ ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) and MASCOT web based search engine. Results: We reported 4 differentially expressed proteins involved in the pathological process of esophageal cancer, such as annexinA1 (ANXA1), peroxiredoxin-2 (PRDX2), transgelin (TAGLN) andactin-aortic smooth muscle (ACTA2). Conclusion: In this report we have introduced new potential biomarker (ACTA2). Moreover, our data confirmed some already known markers for EC in our region.
Keywords :
PROTEOMICS , Peroxiredoxin , Two-dimensional-electrophoresis (2D) , Transgelin , Annexin , Esophageal cancer , Actin-aortic smooth muscle
