Evaluation of callus induction and plant regeneration in Citrullus colocynthis (L.) Schrad Schrad

Abstract In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopurine (BAP) (2 mg/l) and naphthalene acetic acid (NAA) (0.1 mg/l). The findings suggested that capacity of callus generation in BAP and NAA was better than IAA and Kin, but shoot generation and increase of shoot length under IAA and Kin treatment were significant. In BAP and NAA media the callus differentiated in shoot, but generation of shoot and increase in its length was not significant.