Author/Authors :
Fattahi Bafghi، Ali نويسنده , , Sakvand، Tahereh نويسنده Department of Medicinal plants, Kermanshah Jahade-Daneshgahi, Institute of Higher Education, Kermanshah, Iran. , , Hajialyani، Marziyeh نويسنده Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. , , Shahbazi، Behzad نويسنده Department of Mining Engineering, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN , , Shakiba، Mohammad Reza نويسنده , , Tajehmiri، Ahmad نويسنده Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. , , Shakiba، Ebrahim نويسنده Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. ,
Abstract :
Purpose: This study aims to prepare a novel, natural nanoparticle (NP) as a drug carrier, which also has inherent therapeutic effects.
Methods: Pistacia khinjuk gum NPs were prepared and Response surface methodology (RSM) was used for statistical analysis of data and optimizing the size of NPs.
Results: NPs were in the range of 75.85–241.3 nm. The optimization study was carried out, and an optimized size (70.86nm) was obtained using DMSO as a solvent. The volume of the organic phase was 111.25µl, and the concentration of gum was 1% w/v. The cell viability assay was performed on the pure gum and NPs toward β-TC3, MCF7, and HT29 cell lines. It was observed that NPs have higher cytotoxic activity in comparison with pure gum, and that the IC50value was achieved at 1% of NPs in β-TC3 cells. The obtained NPs demonstrated antibacterial activity against two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus).
Conclusion: Altogether, according to the obtained results, these NPs with inherent cytotoxicity and antibacterial activity are an attractive carrier for drug delivery.
