Platelet Transfusion Outcome and Flow Cytometric Monocyte Phagocytic Assay (FMPA)

Abstract Background: 􀀷􀁋􀁌􀁖􀀃􀁖􀁗􀁘􀁇􀁜􀀃􀁚􀁄􀁖􀀃􀁇􀁈􀁖􀁌􀁊􀁑􀁈􀁇􀀃􀁗􀁒􀀃􀁈􀁙􀁄􀁏􀁘􀁄􀁗􀁈􀀃􀁓􀁏􀁄􀁗􀁈􀁏􀁈􀁗􀀃􀁗􀁕􀁄􀁑􀁖􀁉􀁘􀁖􀁌􀁒􀁑􀀃􀁒􀁘􀁗􀁆􀁒􀁐􀁈􀀃􀁙􀁌􀁄􀀃􀃀􀁒􀁚􀀃􀁆􀁜􀁗􀁒􀁐􀁈􀁗􀁕􀁌􀁆􀀃􀁐􀁒􀁑􀁒􀁆􀁜􀁗􀁈􀀃􀁓􀁋􀁄􀁊􀁒􀁆􀁜􀁗􀁌􀁆􀀃􀁄􀁖􀁖􀁄􀁜􀀃􀀋􀀩􀀰􀀳􀀤􀀌􀀑􀀃􀀃 Method: 􀀩􀁌􀁉􀁗􀁈􀁈􀁑􀀃􀁓􀁄􀁗􀁌􀁈􀁑􀁗􀁖􀀃􀁚􀁌􀁗􀁋􀀃􀁄􀀃􀁋􀁌􀁖􀁗􀁒􀁕􀁜􀀃􀁒􀁉􀀃􀁐􀁘􀁏􀁗􀁌􀁓􀁏􀁈􀀃􀁓􀁏􀁄􀁗􀁈􀁏􀁈􀁗􀀃􀁗􀁕􀁄􀁑􀁖􀁉􀁘􀁖􀁌􀁒􀁑􀁖􀀃􀁄􀁑􀁇􀀃􀂿􀁉􀁗􀁈􀁈􀁑􀀃􀁆􀁒􀁑􀁗􀁕􀁒􀁏􀁖􀀃􀁚􀁈􀁕􀁈􀀃􀁈􀁑􀁕􀁒􀁏􀁏􀁈􀁇􀀃􀁌􀁑􀀃􀁗􀁋􀁌􀁖􀀃􀁖􀁗􀁘􀁇􀁜􀀑􀀃􀀦􀀰􀀩􀀧􀀤􀀐􀁏􀁄􀁅􀁈􀁏􀁈􀁇􀀃 􀁓􀁏􀁄􀁗􀁈􀁏􀁈􀁗􀁖􀀃􀁚􀁈􀁕􀁈􀀃􀁌􀁑􀁆􀁘􀁅􀁄􀁗􀁈􀁇􀀃􀁚􀁌􀁗􀁋􀀃􀁓􀁄􀁗􀁌􀁈􀁑􀁗􀁖􀂶􀀃􀁖􀁈􀁕􀁄􀀃􀁄􀁑􀁇􀀃􀁚􀁈􀁕􀁈􀀃􀂿􀁑􀁄􀁏􀁏􀁜􀀃􀁌􀁑􀁆􀁘􀁅􀁄􀁗􀁈􀁇􀀃􀁚􀁌􀁗􀁋􀀃􀁐􀁒􀁑􀁒􀁆􀁜􀁗􀁈􀁖􀀃􀁌􀁑􀀃􀁄􀀃􀁗􀁘􀁅􀁈􀀃􀁄􀁑􀁇􀀃􀁄􀁑􀁄􀁏􀁜􀁝􀁈􀁇􀀃􀁅􀁜􀀃􀃀􀁒􀁚􀀃􀁆􀁜􀁗􀁒􀁐􀁈􀁗􀁕􀁜􀀑􀀃􀀰􀁒􀁑􀁒􀁆􀁜􀁗􀁈􀁖􀀃 that phagocytosed platelets were detected as a CMFDA-positive platelet population via monocyte gate. The FMPA results were compared with CCI results for the patients. Result: The FMPA result correlated with 1-hour (r = -0.885, P = 0.001) and 24-hour (r = -0.884, P􀀃􀀠􀀃􀀓􀀑􀀓􀀓􀀔􀀌􀀃􀀦􀀦􀀬􀀑􀀃􀀷􀁋􀁈􀁕􀁈􀀃􀁌􀁖􀀃􀁄􀀃􀁖􀁌􀁊􀁑􀁌􀂿􀁆􀁄􀁑􀁗􀀃􀁇􀁌􀁉- ference in means of FMPA results between the patients with immune platelet refractoriness (68.46 ± 10.4%), non-refractory group (37.73 ± 15.21%) and the control group (18.27 ± 2.86%). Conclusion: Our data showed that FMPA has good results in evaluation of platelet transfusion outcome and may be useful as an indicator of platelet transfusion response.